Injectable hydrogels based on silk fibroin peptide grafted hydroxypropyl chitosan and oxidized microcrystalline cellulose for scarless wound healing

Scars are consequences of the wound healing process, and eliminating scar formation remains a significant challenge. Here, an injectable HMSC hydrogel was developed based on silk fibroin peptide grafted hydroxypropyl chitosan (HPCS-g-SFP) oxidized microcrystalline cellulose (OMCC) via Schiff base bonds. The synthesized HPCS-g-SFP copolymer displayed efficient free radical scavenging ability hydrogen hydroxyl radicals 1,1-diphenyl-2-picrylhydrazyl (DPPH). pore size, gelling time, equilibrium swelling rate water retention properties could be regulated by changing ratio OMCC HPCS-g-SFP. Then, tetramethylpyrazine (TMP) encapsulated into to obtain TMP-loaded hydrogel. facilitated 95% cell activity after culturing with human skin fibroblasts (HSF) or hypertrophic fibroblast (HSFB) cells for 24 h. Additionally, in vivo animal experiments confirmed that promoted rapid while preventing formation. designed has potentials promoting scarless healing.